Journal: Cell reports
Article Title: TLR9 Sensing of Self-DNA Controls Cell-Mediated Immunity to Listeria Infection via Rapid Conversion of Conventional CD4 + T Cells to T reg .
doi: 10.1016/j.celrep.2020.01.040
Figure Lengend Snippet: Figure 5. Role of TLR9 and IL-12 (p70) in Treg Conversion (A and B) Groups of C57BL/6 and TLR-deficient mice infected with LD or HD Lm DactA-Ova. (A) Absolute number of Ova-specific CD8+ T cells at day 7 post-infection with HD Lm DactA-Ova compared among splenic isolates of Tlr2/, Tlr4/, Tlr7/, and Tlr9M7Btlr mice (n = 6–10 per group). (B) Absolute number of Ova-specific CD8+ T cells at day 7 in the spleens of Tlr9M7Btlr mice ± CD4+ T cell depletion (n = 6–8 per group). (C and D) Groups of C57BL/6 and Tlr9M7Btlr mice systemically infected with HD Lm DactA-Ova. (C) Fold change in serum cytokines and chemokines from 0–6 h post-infection. (D) Selected serum cytokine/chemokine concentration at 6 h post-infection displaying significance in (C) (n = 6 per group). (legend continued on next page)
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains WT Listeria monocytogenes (Lm) 10403S Daniel A. Portnoy (University of California, Berkeley, CA) N/A WT Lm Ova Daniel A. Portnoy (University of California, Berkeley, CA) N/A Lm DactA 10403S Daniel A. Portnoy (University of California, Berkeley, CA) N/A Lm DactA-Ova 10403S Daniel A. Portnoy (University of California, Berkeley, CA) N/A Chemicals, Peptides, and Recombinant Proteins CellTrace Violet (CTV) Life Technologies Cat#C34557 4’,6-diamidino-2-phenylindole (DAPI) Molecular Probes Cat#D1306 diphtheria toxin (DT) Sigma-Aldrich Cat#D0564 DNase I, from bovine pancreas Sigma-Aldrich Cat#11284932001 H-2Kb-Ova iTAg tetramer PE MBL International Cat#TB-5001-1 Mouse T-Activator anti-CD3/CD28 Dynabeads GIBCO Cat#11452D Ova257-264 peptide (SIINFEKL) AnaSpec Cat#AS-60193-1 rmIL-12 (p70) R&D Systems Cat#419-ML-010 rmTGF-b1 R&D Systems Cat#7666-MB-005 Critical Commercial Assays human/mouse TGF-b1 ELISA Invitrogen Cat#88-8350-86 liquid ALT (SGPT) Reagent set Pointe Scientific Cat#A7526150 live/Dead Fixable Yellow Dead Cell Stain kit Invitrogen Cat#L-34968 MILLIPLEX MAP 32-plex mouse cytokine/chemokine panel EMD Millipore Cat#MCYTMAG-70K-PX32 mouse IFN-a2/4 Platinum ELISA eBioscience Cat#BMS6027 mouse IFN-b VeriKine ELISA PBL Assay Science Cat#42400 Novagen NovaQUANT Mouse Mitochondrial to Nuclear Ratio kit EMD Millipore Cat#72621-1KIT Deposited Data RNA-Seq metadata NCBI gene expression omnibus GSE106554 Experimental Models: Mice C57BL/6 The Jackson Laboratory Stock#000664 Act-mOva H-2kb / Marc K. Jenkins (University of Minnesota, Minneapolis, MN) and (Feau et al., 2011; Janssen et al., 2005) N/A Batf3 / The Jackson Laboratory Stock#013755 Foxp3EGFP (FoxP3-EGFP) The Jackson Laboratory Stock#006772 Foxp3DTR-EGFP The Jackson Laboratory Stock#016958 Il12rb2 / The Jackson Laboratory Stock#003248 Nr4a1GFP (Nur77-GFP) Kristin A. Hogquist (University of Minnesota) and (Moran et al., 2011) N/A p65-GFP Manolis Pasparakis (University of Cologne, Cologne, Germany) and (De Lorenzi et al., 2009) N/A Tlr2 / The Jackson Laboratory Stock#004650 Tlr4 / The Jackson Laboratory Stock#007227 Tlr7 / The Jackson Laboratory Stock#008380 Tlr9M7Btlr The Jackson Laboratory Stock#014534 (Continued on next page) Cell Reports 31, 107249, April 7, 2020 e2
Techniques: Infection, Concentration Assay
